TDM of Immunosuppressants in Whole Blood in 8 Seconds Using LDTD-MS/MS

Introduction

Everolimus, sirolimus, tacrolimus, and cyclosporine-A are frequently used to prevent solid organ transplant rejection in recipients. However, these immunosuppressants have narrow therapeutic concentration windows and display considerable variability in their pharmacokinetics both within and between patients. As a result, prompt feedback of blood sample results is essential. We suggest employing the Axino Ion Source, utilizing LDTD (Laser Diode Thermal Desorption) process, in combination with a mass spectrometer, to enable ultra-fast quantification. This approach would facilitate accurate measurement through a reference calibration curve.

Presented at ASMS 2025.