High-Throughput Analysis of Testosterone and DHEA in Human Serum

Introduction

The determination of serum testosterone and dehydroepiandrosterone (DHEA) concentrations facilitates the diagnosis and treatment of various diseases. In addition to testosterone, the primary androgen hormone, many other androgenic hormones play an important role in the diagnosis and treatment of various diseases. Since the introduction of steroid immunoassays, antibody affinity-based methods have been widely used to measure bioactive steroids in biological fluids due to their high sensitivity. However, these techniques are limited by the specificity of antibodies for epitopes, which may lead to cross-reactions with structurally similar steroids, thereby reducing the specificity of the method. To obtain a rapid analytical method to separate testosterone and DHEA, isobaric compound, a specific APCI ionisation and extraction method and an LDTD-MS/MS analytical method was developed.

Presented during ASMS 2025.