FAQ Series: What is the Typical Sample Preparation to Use on a Luxon Ion Source®?

Back in July, we started answering the most frequently asked questions we have at Phytronix.

As you may now know, when using the Luxon Ion Source®, it is imperative to use LazWell plates. Given that the samples have to be dried before they enter the Luxon Ion Source, often times our clients ask us if their samples require a special treatment.

The short answer is: Not at all!

The nuanced answer would be: It depends on your own application and on your matrix. The typical sample preparation methods are valid with the Luxon Ion Source-MS system as they would be for a traditional LC-MS, but each matrix reacts differently and cannot be treated the same way.

In order to make it easier, we decided to prepare a table that can guide you in knowing what methods have been tested and work on different types of matrices:

Plasma samples: A protein precipitation extraction is usually done with acetonitrile to remove the proteins from the sample. After the centrifugation, the supernatant is deposited into the LazWell plate.

Food samples, to increase sensitivity or reduce noise: In this case, a liquid-liquid extraction (LLE) will be preferred. Again, with this type of extraction, the upper layer would be deposited into the LazWell plate. Don’t worry our extractions methods are usually in the 1-3 mL range and never require a separation funnel!

Drug Discovery or simple matrix: A dilution is often used with these types of applications.

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