Back in July, we started answering the most frequently asked questions we have at Phytronix.
As you may now know, when using the Luxon Ion Source®, it is imperative to use LazWell plates. Given that the samples have to be dried before they enter the Luxon Ion Source, often times our clients ask us if their samples require a special treatment.
The short answer is: Not at all!
The nuanced answer would be: It depends on your own application and on your matrix. The typical sample preparation methods are valid with the Luxon Ion Source-MS system as they would be for a traditional LC-MS, but each matrix reacts differently and cannot be treated the same way.
In order to make it easier, we decided to prepare a table that can guide you in knowing what methods have been tested and work on different types of matrices:
Plasma samples: A protein precipitation extraction is usually done with acetonitrile to remove the proteins from the sample. After the centrifugation, the supernatant is deposited into the LazWell™ plate.
Food samples, to increase sensitivity or reduce noise: In this case, a liquid-liquid extraction (LLE) will be preferred. Again, with this type of extraction, the upper layer would be deposited into the LazWell™ plate. Don’t worry – our extractions methods are usually in the 1-3 mL range and never require a separation funnel!
Drug Discovery or simple matrix: A dilution is often used with these types of applications.
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