Ethyl glucuronide (EtG) testing is tricky – who hasn’t seen a movie or a show where they depict a character diluting their urine with toilet water or apple juice? Although this direct metabolite of ethanol can be quantified in hair and blood, urine is still the best and most reliable by-product of the human metabolism when it comes to alcohol detection, since it permits the detection of EtG for several hours after ingestion. To bypass these trickeries, a biomarker found in urine needs to be quantified to ensure the integrity of the sample.
For our study, creatinine, the product broken down from creatine phosphate in muscle, was chosen. The concentration of creatinine in urine varies from one person to another, depending on muscle mass, sex, age and kidney function, but it still stays in a measurable and specific range for each sex: women – 290-2260 mg/L, men – 400-2780 mg/L. Simply put, if the sample is diluted, the creatinine levels in urine will show it.
The poster that will be presented at SOFT 2018 quantifies creatinine using an isotope dilution analysis approach and can then quantify ethyl glucuronide effectively. One of our research scientists will be at the tradeshow to answer all of your questions regarding this application.
Visit us at our booth #506 at the Annual SOFT Meeting in Minneapolis.