Using PAL System Robots with the LDTD® Technology

In a typical chromatographical analysis, the most time-consuming step is, by far, sample preparation. It is estimated that 61% of the time is consumed by it, while data management, data collection and data analysis respectively make up 27%, 6% and 6% of the time.1

Consequently, sample preparation is the step that generates the most errors during a chromatographical analysis1. The below pie chart demonstrates it:

The PAL RTC model is a complete sample preparation tool that can be used for dilutions, liquid-liquid and solid phase extractions as well as for protein precipitations. With the right modules, it can also derivatize, mix and refrigerate samples. It also helps minimize contamination and mix-ups during manual sample preparations. This platform can be used in combination with the LC, GC and LDTD® technologies to analyze different types of samples.

A simple preparation example using Dextrorphan and Dextromethorphan in plasma samples is presented in application note:

AN 1402 – On-line automated protein precipitation preparation followed by LC-MS/MS analysis and LDTD-MS/MS cross validation

With the Luxon Ion Source® and the LDTD, the RTC is mostly used to prepare blood samples with DBS (dried blood spot) In-Tips.
It has enabled us to: 

  • Compare micro sampling to standard volume protein precipitations;
  • Evaluate the hematocrit effect when using DBS;
  • Demonstrate the effectiveness of dried blood spot fabric deposited into tips.
PAL RTC with extended x-axis for LC/MS

PAL RTC with extended x-axis for LC/MS

 

Here are a few applications in which different RTC modules are used: 

In tips micro-sampling comparison with standard volume plasma protein precipitation with an on-line sample preparation system 

Fully automated evaluation of Dextrorphan extraction procedure by DBS In-Tips, analyzed with RTC-LC-MS/MS 

Hematocrit effect: Does it influence the analysis of plasma samples or is it just a DBS concern

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