In a typical chromatographical analysis, the most time-consuming step is, by far, sample preparation. It is estimated that 61% of the time is consumed by it, while data management, data collection and data analysis respectively make up 27%, 6% and 6% of the time.1
Consequently, sample preparation is the step that generates the most errors during a chromatographical analysis1. The below pie chart demonstrates it:
The PAL RTC model is a complete sample preparation tool that can be used for dilutions, liquid-liquid and solid phase extractions as well as for protein precipitations. With the right modules, it can also derivatize, mix and refrigerate samples. It also helps minimize contamination and mix-ups during manual sample preparations. This platform can be used in combination with the LC, GC and LDTD® technologies to analyze different types of samples.
A simple preparation example using Dextrorphan and Dextromethorphan in plasma samples is presented in application note:AN 1402 – On-line automated protein precipitation preparation followed by LC-MS/MS analysis and LDTD-MS/MS cross validation
With the Luxon Ion Source® and the LDTD, the RTC is mostly used to prepare blood samples with DBS (dried blood spot) In-Tips.
It has enabled us to:
- Compare micro sampling to standard volume protein precipitations;
- Evaluate the hematocrit effect when using DBS;
- Demonstrate the effectiveness of dried blood spot fabric deposited into tips.
Here are a few applications in which different RTC modules are used: